خاصیت ضدباکتریایی عصاره متانولی چهار گونه گلسنگ بومی ایران‌

نوع مقاله: پژوهشی

نویسندگان

1 دانشگاه آزاد اسلامی واحد میانه باشگاه پژوهشگران جوان و نخبگان میانه، ایران.

2 استادیار سازمان پژوهش های علمی و صنعتی ایران، تهران، ایران.

3 دانشگاه آزاد اسلامی واحد میانه ، میانه، ایران.

چکیده

چکیدهگلسنگ­ها به عنوان یکی از منابع سرشار از ترکیبات طبیعی با خاصیت آنتی­بیوتیک شناخته شده اند که از برخی از آنها به عنوان دارو و برای درمان بیماری­های خاص استفاده می­شود. در این پژوهش اثر بازدارندگی، باکتری­ایستایی و کشندگی عصاره متانولی چهار گونه گلسنگ جمع­آوری شده از منطقه ارسباران، آذربایجان شرقی شامل Pleopsidiumgobiensis، Parmelinatiliacea، Anaptychiasetifera و Lecanoraargopholis روی چند گونه باکتری گیاهی از جمله Bacillus subtilis، Pseudomonas fluorescens، Enterobactersp. در آزمایشگاه با روشدیسک­گذاری و تعیین حداقل غلظت بازدارنده و کشنده مورد بررسی قرار گرفت. در روش نشر در آگار، عصاره­های متانولی گلسنگ­های مورد بررسی اثر بازدارنده قابل توجهی بر رشد باکتری­ها به جز Enterobacter sp. داشتند. عصاره­های گلسنگ A. setifera از بازدارندگی کمتری در مقایسه با سایرین بر رشد باکتری­های مورد مطالعه داشت. عصاره متانولی همه گلسنگ­ها کم و بیش بر باکتری­های مورد مطالعه اثر بازدارنده و کشنده داشتند. گلسنگ­های P. gobiensis و P. tiliacea اثر باکتری­ایستایی و نیز کشندگی بسیار بیشتری در مقایسه با دو گلسنگ دیگر نشان دادند. عصاره­های گلسنگی برابر B. subtilisبیشتر خاصیت بازدارندگی داشتند. در روش حداقل غلظت باکتری­ایستایی و کشندگی، واکنش باکتری­کشی و باکتری­ایستایی Enterobacter sp. از دو باکتری دیگر به عصاره­های گلسنگی بیشتر بود. با توجه به نتایج این مطالعه به نظر می­رسد عصاره­های گلسنگی از کارآیی قابل ملاحظه­ای برای مقابله باکتری­های گیاهی مورد مطالعه برخوردارند.

کلیدواژه‌ها


عنوان مقاله [English]

Antibacterial activity of methanol extract of some Iranian lichens

نویسندگان [English]

  • Soleiman Jamshidi 1
  • Seyyedeh Maryam Shahidi 1
  • Mohammad Sohrabi 2
  • Samira Jamshidi 3
1 Young Researchers and Elite Club Miyaneh Branch Islamic Azad University Miyaneh, Iran
2 Professor assistant of Iranian Research Organization for Science and Technology (IROST) Tehran, Iran
3 Islamic Azad University Miyaneh, Iran
چکیده [English]

Lichens are known as one of the greatest sources of natural compounds having antibiotic properties which some of them are being used as drugs for special diseases therapy. In current study, inhibitive, bacteriostatic and bactericidal activities of methanol extracts of five lichens derived from Arasbaran, East Azarbaijan Province of Iran including Pleopsidiumgobiensis, Parmelinatiliacea, Anaptychiasetifera and Lecanoraargopholis on some plant derived bacteria such asPseudomonas fluorecens, Bacillus subtillis and Enterobactersp. were studied using disc diffusion and minimal inhibitory and bactericide concentration methods. Methanol extracts of lichens had significant inhibitive effects of studied bacteria other than Enterobactersp. in disc diffusion agar method. A. setifera extracts had less inhibitive in bacteria than others. All lichens extracts had more or less bacteriostatic and bactericidal effects on bacteria. Methanol extracts of all lichens had various bacteriostatic and bactericidal effects on bacteria P. gobiensis and P. tiliaceahad much more bacteriostatic and bactericide comparing two other lichens. Lichens extracts was more inhibitive against B. subtillis. The bacteriostatic and bactericidal reaction of Enterobactersp. to lichen extracts was more than two other bacteria. Regarding results of the results, the lichens extracts could have remarkable potential for studied plant bacteria biocontrol and might be considered as promising agents against pathogens.

کلیدواژه‌ها [English]

  • biocontrol
  • antibacterial activity
  • Natural compounds
  • antimicrobials
  • bacteriostatic
  • bactericide
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