1دانشگاه آزاد اسلامی واحد میانه باشگاه پژوهشگران جوان و نخبگان میانه، ایران.
2استادیار سازمان پژوهش های علمی و صنعتی ایران، تهران، ایران.
3دانشگاه آزاد اسلامی واحد میانه ، میانه، ایران.
چکیده
چکیدهگلسنگها به عنوان یکی از منابع سرشار از ترکیبات طبیعی با خاصیت آنتیبیوتیک شناخته شده اند که از برخی از آنها به عنوان دارو و برای درمان بیماریهای خاص استفاده میشود. در این پژوهش اثر بازدارندگی، باکتریایستایی و کشندگی عصاره متانولی چهار گونه گلسنگ جمعآوری شده از منطقه ارسباران، آذربایجان شرقی شامل Pleopsidiumgobiensis، Parmelinatiliacea، Anaptychiasetifera و Lecanoraargopholis روی چند گونه باکتری گیاهی از جمله Bacillus subtilis، Pseudomonas fluorescens، Enterobactersp. در آزمایشگاه با روشدیسکگذاری و تعیین حداقل غلظت بازدارنده و کشنده مورد بررسی قرار گرفت. در روش نشر در آگار، عصارههای متانولی گلسنگهای مورد بررسی اثر بازدارنده قابل توجهی بر رشد باکتریها به جز Enterobacter sp. داشتند. عصارههای گلسنگ A. setifera از بازدارندگی کمتری در مقایسه با سایرین بر رشد باکتریهای مورد مطالعه داشت. عصاره متانولی همه گلسنگها کم و بیش بر باکتریهای مورد مطالعه اثر بازدارنده و کشنده داشتند. گلسنگهای P. gobiensis و P. tiliacea اثر باکتریایستایی و نیز کشندگی بسیار بیشتری در مقایسه با دو گلسنگ دیگر نشان دادند. عصارههای گلسنگی برابر B. subtilisبیشتر خاصیت بازدارندگی داشتند. در روش حداقل غلظت باکتریایستایی و کشندگی، واکنش باکتریکشی و باکتریایستایی Enterobacter sp. از دو باکتری دیگر به عصارههای گلسنگی بیشتر بود. با توجه به نتایج این مطالعه به نظر میرسد عصارههای گلسنگی از کارآیی قابل ملاحظهای برای مقابله باکتریهای گیاهی مورد مطالعه برخوردارند.
Antibacterial activity of methanol extract of some Iranian lichens
نویسندگان [English]
Soleiman Jamshidi1؛ Seyyedeh Maryam Shahidi1؛ Mohammad Sohrabi2؛ Samira Jamshidi3
1Young Researchers and Elite Club
Miyaneh Branch
Islamic Azad University
Miyaneh, Iran
2Professor assistant of
Iranian Research Organization for Science and Technology (IROST)
Tehran, Iran
3Islamic Azad University
Miyaneh, Iran
چکیده [English]
Lichens are known as one of the greatest sources of natural compounds having antibiotic properties which some of them are being used as drugs for special diseases therapy. In current study, inhibitive, bacteriostatic and bactericidal activities of methanol extracts of five lichens derived from Arasbaran, East Azarbaijan Province of Iran including Pleopsidiumgobiensis, Parmelinatiliacea, Anaptychiasetifera and Lecanoraargopholis on some plant derived bacteria such asPseudomonas fluorecens, Bacillus subtillis and Enterobactersp. were studied using disc diffusion and minimal inhibitory and bactericide concentration methods. Methanol extracts of lichens had significant inhibitive effects of studied bacteria other than Enterobactersp. in disc diffusion agar method. A. setifera extracts had less inhibitive in bacteria than others. All lichens extracts had more or less bacteriostatic and bactericidal effects on bacteria. Methanol extracts of all lichens had various bacteriostatic and bactericidal effects on bacteria P. gobiensis and P. tiliaceahad much more bacteriostatic and bactericide comparing two other lichens. Lichens extracts was more inhibitive against B. subtillis. The bacteriostatic and bactericidal reaction of Enterobactersp. to lichen extracts was more than two other bacteria. Regarding results of the results, the lichens extracts could have remarkable potential for studied plant bacteria biocontrol and might be considered as promising agents against pathogens.
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