اثر تنش خشکی ناشی از پلی‌اتیلن‌گلایکول بر خصوصیات مورفولوژیک، فیزیولوژیک و بیوشیمیایی ارقام انتخابی بادام

نوع مقاله: پژوهشی

نویسندگان

1 دانشجوی دکتری گروه علوم باغبانی، دانشگاه گیلان رشت، ایران

2 دانشجوی کارشناسی ارشد گروه علوم باغبانی دانشکده کشاورزی دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران تهران، ایران

3 دانشیار موسسه نهال و بذر کرج تهران، ایران

4 دانشجوی کارشناسی ارشد گروه علوم باغبانی دانشگاه آزاد اسلامی واحد کرج کرج، ایران

چکیده

این تحقیق در ایستگاه تحقیقات باغبانی موسسه اصلاح و تهیه نهال و بذر کرج در قالب طرح پایه­ کاملاً تصادفی به صورت فاکتوریل انجام شد. فاکتورهای آزمایش شامل بذور ارقام مختلف بادام (سوپرنووا، پرلیس، 99D-، مارکونا، ربیع و 16-k) و تنش اسمزی ناشی از پلی­اتیلن­گلایکول در پنج سطح 0، 2-، 4-، 6- و 8- بار بود. با اعمال تنش خشکی روی گیاهان ارتفاع گیاه، تعداد برگ، کلروفیل a، b و کلروفیل کل، میزان گلوکز و فروکتوز، محتوای رطوبت نسبی برگ، کاهش و میزان پرولین و نشت یونی افزایش یافت ولی میزان این کاهش و یا افزایش در بین ارقام بررسی شده با یکدیگر اختلاف معنی­داری نشان دادند. همچنین، رقم پرلس و K66 بیشترین حساسیت را نسبت به تنش خشکی از خود نشان دادند و تعداد برگ و ارتفاع گیاه در تنش اسمزی 2- بار نیز به طور معنی­داری نسبت به شاهد کاهش نشان داد. بیشترین مقاومت به تنش خشکی در ارقام D99 و مارکونا مشاهده شد و تنش اسمزی تا 6- بار را تحمل نمودند و تنها در تنش اسمزی 8- بار تعداد برگ و ارتفاع آن به طور معنی­داری نسبت به گیاهان شاهد کاهش یافت. نتایج حاصل از بررسی صفات فیزیولوژیک و بیوشیمیایی با نتایج حاصل از
اندازه­گیری صفات رویشی همخوانی داشت. ارقام مارکونا و D99 که بیشترین میزان تولید پرولین و کمترین میزان نشت یونی را دارا بودند، به عنوان مقاوم­ترین ارقام نسبت به تنش خشکی تشخیص داده شده و رقم K66 به عنوان حساس­ترین رقم به تنش خشکی معرفی شد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of drought stress by polyethylene glycol on morphological, physiological and biochemical characteristics on selected cultivars almond

نویسندگان [English]

  • Ali Momenpour 1
  • Hosein Mohammadi 2
  • Ali Imani 3
  • Somayeh Mohammadian 4
1 PhD student University of Guilan Guilan Rasht, Iran Email
2 Master student Islamic Azad University Tehran, Iran
3 Associate professor Seed and Plant Institute Karaj, Iran
4 Master student Islamic Azad University Karaj, Iran
چکیده [English]

This study during the year of 2011 and 2012 in horticultural research station and seed and plant improvement Institute, Karaj as factorial on the base completely randomized design was done. Factors include almond seedlings tested in 6 levels (supernova, Perlis, D 124, Marcona, Rabie, and k1-16) and osmotic stress of polyethylene glycol in 5 levels (0, 2-, 4-, -6 and 8-bar). The results showed that with drought stress on plants, plant height, number of leaves, the amount of chlorophyll a, chlorophyll b and total chlorophyll, the amount of glucose and fructose content, relative humidity content decreased and the amount of proline and the rate of ion leakage increased, but increase or reduce these traits in the cultivars with each other had significant differences. The results of investigation growth traits showed that Peerless and k66 cultivars had the most sensitivity to the drought so that the number of these plants leaves and their height under osmotic stress -2 bar are reduced in comparative with plant control significantly. The most resistance to drought was shown in D-124 and Marcona cultivars so that osmotic stress could endure up to -6 bars and only in osmotic stress -8 bars, the number of leaves and their height was significantly reduced compared with control plants. The results from physiological and biochemical characteristics were very similar with the results of the measurements on morphological traits. D-124 and Marcona cultivars had the highest level of production proline and the lowest ion leakage. They were diagnosed as the most resistance to drought stress, while cultivar k16 had the most sensitive to drought stress.

کلیدواژه‌ها [English]

  • Drought stress
  • glucose and fructose
  • relative water humidity
  • Proline
  • Ion leakage
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