نوع مقاله: پژوهشی
نویسندگان
1 استادیار گروه گیاهپزشکی، دانشکده کشاورزی دانشگاه آزاد اسلامی واحد تاکستان، ایران،
2 کارشناس ارشد اصلاح نباتات، گروه بیماری شناسی گیاهی دانشکده کشاورزی و منابع طبیعی دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Fusarium wilt causes cosiderable yield loss in most tomato-growing regions around the world. The best way to control this disease is using tomato resistant cultivars. Molecular markers linked to resistance gene would be useful for tomato improvement programs. In this study, a cleaved amplified polymorphic sequence (CAPS) marker (TAO1902) was used to identify tomato cultivars possessing I-2 gene, which confers resistance to Fusarium oxysporum f. sp. lycopersici race 2. DNA was extracted using CTAB method from 27 hybrids and commercial tomato varieties. To evaluate existence of I-2 gene in tomato cultivars, PCR-RFLP was down using RsaI and FokI restriction enzymes. An RsaI-digested fragment of 500 bp and two restriction fragments of 390 and 410 bp for FokI digestion of TAO1902 were revealed in the resistant varieties. Results showed that 14 out of 27 tomato hybrids and cultivars were homozygous resistant and the other 13 hybrids were susceptible to fusarium wilt. Pathogenicity test showed that there were no symptoms in resistance gene I-2 amplifing tomato varieties. However, tomato varieties with no resistance band produced disease symptoms with different disease intensities. Results showed that 7 out of 27 tomato cultivars and hybrids, and 7 hybrids were resistant to F. oxysporum f. sp. lycopersici race 2 and so, these cultivars and hybrids are recommended in regions that race 2 is dominant.
کلیدواژه‌ها [English]
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1994). Current protocols in molecular biology. John Wiley and Sons Inc: New York.
Barone A (2003) Molecular marker-assisted selection for resistance to pathogens in tomato. International Workshop of Molecular Biology 2003, October 17-18, Villa Guliano, Torino, Italy, 29-34.
Booth C (1971) The genus Fusarium. Commonwealth Mycological Institute, Kew, Surrey, England.
Hamelin R, Ouellette GB, Bernier L (1993) Identification of Gremmenie laabietina races with random amplified polymorphic DNA markers. Applied and Environmental Microbiology 59: 1752-1755.
Hirano Y, Arie T (2006) PCR-based differentiation of Fusarium f. sp. lycopersici and radicis lycopersici and races of F. oxysporum f. sp. lycopersici. Japanese Journal of General Plant Pathology 72: 273-283.
Hulbert SH, Webb CA, Smith SM, Sun Q (2001) Resistance gene complexes: evolution and utilization. Annual Review of Phytopathology 39: 285-312.
Garland S, Sharman M, Persley AD, Mc Grath D (2005) The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato. Australian Journal of Agricultural Research 56: 285-289.
Gerlach W, Nirenberg H (1982) The genus Fusarium: A pictorial atlas. Mitteilungen aus der Biologischen Bundesanstalt Für Land- und Forstwirtschaft (Berlin-Dahlem)209: 1-405.
Goodwin PH, Annis SL (1991) Rapid identification of genetic variation and pathotype of Leptosphaeria maculans by random amplified polymorphic DNA assay. Applied and Environmental Microbiology 57: 2482-2486.
Grattidge R, Obrien RG (1982) The occurrence of a third race of Fusarium wilt of tomato in Queensland. Plant Disease66: 165-166.
Kim JT, Park H, Hahm Y, Hun Yu S (2001) Crown and root rot of greenhouse tomato caused by Fusarium oxysporum f. sp. radicis-lycopersici in Korea. Plant Pathology Journal 17(5): 290-294.
Mago R, Brown-Guedira G, Dreisigacker S, Breen J, Jin Y, Singh R, Appels R, Lagudah ES, Ellis J, Spielmeyer W (2010) An accurate DNA marker assay for stem rust resistance geneSr2 in wheat. Theoretical and Applied Genetics 122(4): 735-744.
Malhotra SK, Vashistha RN (1993) Genetics of resistance to Fusarium wilt race 1 in current tomato (Lycopersicon pimpinellifolium). Indian Journal of Agricaltural Science 63: 246-347.
Mc Grath DJ, Gillespie G, Vawdrey L (1987) Inheritance of resistance to Fusarium oxysporum f. sp. lycopersici races 2 and 3 in Lycopersicon pennellii. Australian Journal of Agricaltural Research 38: 729-733.
Stall RE, Walter JM (1965) Selection and inheritance of resistance in tomato to isolates of races 1 and 2 of the Fusarium wilt organism. Phytopathology 55: 1213-1215.
Sedlacek T, Marik P, Chrpova J (2010) Development of CAPS marker for identification of rym4 and rym5 alleles conferring resistance to the Barley Yellow Mosaic Virus complex in barley. Czech Journal of Genetics and Plant Breeding 46(4): 159-163.
Shi A, Vierling R, Grazzini R, Chen P, Caton H, Panthee D (2011) Identification of molecular markers for Sw-5 gene of tomato spotted wilt virus resistance. American Journal of Biothecnology and Molecular Science 1(1): 8-16.
Smith OP, Peterson GL, Beck RJ, Schaad NW, Bonde MR (1996) Development of a PCR-based method for identification of Tilletia indica, causal agent of Karnal bunt of wheat.Phytopathology 86: 115-122.
Staniazsek M, Kozik EU, Marczewski W (2007). A CAPS marker TAO1902 diagnostic for the I-2 gene conferring resistance to Fusarium oxysporum f. sp. lycopersici race 2 in tomato. Plant Breeding 126(3): 331-333.
Stevens MA, Rick CM (1986) Genetics and breeding. In: Atherton JG and Rudich J (Eds), The Tomato Crop. Chapman & Hall, London. pp. 35-109.
Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Martin S, Messeguer GB, Miller RJC, Paterson AH, Pineda O, Ro MS, Wing RA, Wu W, Young ND (1992) High density molecular linkage maps of the tomato and potato genomes. Genetics 132: 1141-1160.
Tanyolac B, Akkale C (2010) Screening of resistance genes to fusarium root rot and fusarium wilt diseases in F3 family lines of tomato (Lycopersicon esculentum) using RAPD and CAPs markers. African Journal of Biotechnology 9(19): 2727-2730
Validov SZ (2007) Biocontrol of tomato foot and root rot in stonewool by Pseudomonas putida strain PCL1760 in a certified greenhouse under industrial conditions. Thesis Validov. تکمیل از اینترنت
Yeam I, Cheorl Kang B, Lindeman W, Frantz J, Faber N, Jahn M (2005) Allele-specific CAPS markers based on point mutations in resistance alleles at the pvr1 locus encoding eIF4E in Capsicum. Theoretical and Applied Genetics 112: 178–186.
Yu Kuklev M, Fesenko IA, Karlov GI (2009) Development of a CAPS marker for the Verticillium wilt resistance in tomatoes. Russian Journal of Genetics 45(5): 575-579.
Zhang AW, Hartman GL, Curio-penny B, Pedersen WL, Becker KB (1999) Molecular detection of Diaporthe phaseolorum and Phomopsis longicolla from soybean seeds. Phytopathology 89: 796-804.
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