اثر پروبیوتیک (پروتکسین) روی عملکرد، فراسنجه‏های سرم و کیفیت تخم‏مرغ در مرغ‏های تخم‏گذار

نوع مقاله: پژوهشی

نویسندگان

عضو هیأت علمی گروه علوم دامی دانشگاه آزاد اسلامی واحد مراغه

چکیده

آزمایشی برای مطالعه اثرات سطوح مختلف پروبیوتیک روی تولید، کیفیت تخم‏مرغ، پارامترهای بیوشیمیایی خون و کلسترول و تری گلیسیرید زرده تخم‏مرغ انجام گرفت. آزمایش با 256 قطعه مرغ تخم‏گذار از سن 32-42 هفتگی در قالب یک طرح کاملاً تصادفی که به چهار تیمار و تکرار و شانزده پرنده در هر تکرار تقسیم شده بود انجام گرفت. مرغ‏ها جیره‏های آزمایشی شامل سطوح صفر، 25، 50 و 75 میلی‏گرم پروبیوتیک (پروتکسین) در هر کیلوگرم جیره دریافت کردند. پروبیوتیک مورد استفاده در این تحقیق پروتکسین تجاری با cfu g-1  109×2 ارگانیسم در محصول بود. تولید تخم مرغ، وزن تخم مرغ، خوراک مصرفی، ضریب تبدیل خوراک و توده تخم مرغ با افزودن پروبیوتیک تحت تأثیر واقع نشد. به هر حال واحد هاو، وزن مخصوص، وزن پوسته تخم مرغ و ضخامت آن و نسبت وزن پوسته به سطح آن تحت تأثیر پروبیوتیک قرار نگرفت. داده‏های مربوط به فراسنجه‏های بیوشیمیایی نشان داد که بین گروه‏های مختلف آزمایشی از نظر مقادیر گلوکز، آلبومین، پروتئین کل، فسفر، کلسیم، تری‏گلیسرید، کلسترول سرم خون تفاوت معنی‏داری وجود ندشت. هم‏چنین اثر پروبیوتیک بر کلسترول و تری گلیسرید زرده تخم مرغ نیز معنی‏دار نبود. با توجه به اطلاعات حاصله نتیجه‏گیری شد که مکمل پروبیوتیک تا سطح 75 میلی‏گرم در کیلوگرم جیره مرغان تخم‏گذار اثرات مفیدی بر عملکرد و صفات کیفی تخم مرغ ندارد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Probiotic (Protexin) on performance, blood biochemical parameters and egg quality in laying hens

نویسندگان [English]

  • Ali Reza Safamehr
  • Ali Nobakht
Scientific Board of Animal Science Department, Islamic Azad University, Maragheh Branch
چکیده [English]

The effect of probiotic on egg production, egg quality, yolk cholesterol, triglyceride and blood biochemical parameters of layer hens was studied. Experiment was carried out with 256 bird and each sixteen bird were considered as a replicate that were randomly allotted to basal with Probiotic supplementation at rates of 25, 50 and 75 mg/ kg diets. Treatments were arranged in a completely randomized design. The Probiotic used in the study was a commercial preparation (Protexin) with 3× 109 spore g-1 of the product. Egg production, egg weight, feed intake, feed conversion ratio and egg mass were not influenced by Probiotic, as well as Haug unit, specific gravity, egg shell weight, egg shell thickness and shell weight/ surface area. Experimental groups had not statistical differences in biochemical parameters such as albumin, total protein, phosphorus, calcium, cholesterol, triglyceride and glucose amounts. Probiotic had not also any effect on egg yolk cholesterol and triglyceride. It was concluded that dietary Probiotic supplementation up to 75 mg/kg of diet had not beneficial effects on the performance and egg quality of laying hens.

کلیدواژه‌ها [English]

  • Probiotic
  • egg quality
  • Cholestrol
  • blood
  • laying hens

1 - آزادگان مهر، م.، م. شمس شرق، ب. دستار  و س. حسنی. 1386. تأثیر سطوح مختلف پروتئین و پروتکسین بر عملکرد جوجه‏های گوشتی. مجله علوم کشاورزی و منابع طبیعی، جلد 14، شماره سوم، 120-110.

  1. Abdulrahim, S. M., M. S. Y. Haddadin and A. R. Hashlamoun. 1996. The influence of Lactobacillus acidophilus and bacitracin on layer performance and cholesterol of plasma and egg yolk. British Poultry Science 37: 341-346.
  2. Andine, W. E., K. S. Muralidara, P. R. Elliker and D. G. England. 1972. Lactic acid bacteria in food and health: A review with special reference to enteropathogenic Escherichia coli as well as certain enteric diseases and their treated with antibiotics and lactobacilli. Journal of Milk Food Technology 35: 691-702.
  3. Baba, E., S. Nagaishi, T. Fukata and A. Arakawa. 1991. The role of intestinal microflora on the prevention of salmonella colonization in gnotobiotic chickens. Poultry Science 70: 1902-1907.
  4. Barrow, P. A. 1992. Probiotics for chickens. In: R. Fuller (ed.), Probiotics: the Scientific Basis Chapman and Hall London, UK, 225-257.
  5. Bartov, I. 1992. Lack of effect of dietary energy and-to-protein ratio and energy concentration on the response of chickens to Virginiamycin. British Poultry Science 33: 381-391.
  6. Cerniglia, G. J., A. C. Goodling  and J. A. Hebert. 1983. The response of layers to feeding Lactobacillus fermentation product. Poultry Science 62: 1399.
  7. Chen, Y. C., and T. C. Chen. 2003. Effects of commercial probiotic of size and quality of hens egg. Poultry Science 82 (1): 330 (Abstract).
  8. Damron, B. L., A. R. Eldered and R. H. Harms. 1976. An improvement in interior egg quality by the feeding of brewers dried grains. Poultry Science 55: 1365-1366.

10.Folch, J., M. Less and G. H. Solane Stanley. 1956. A simple method for the isolation and purification of total lipid from animal tissues. Journal Biochemical chemistry 226:497-509.

11.Fuller, R. 1973. Ecological studies on the Lactobacillus flora associated with the crop epithelium of the fowl. Journal of Applied Bacteriology 36: 131-139.

12.Fuller, R. 1989.  Probiotics in mans and animals. Journal of Applied Bacteriology 66: 365-378.

13.Gallazzi, D., A. Giardini, M. Grazia Mangiagalli, S. Marelli, V. Ferrazzi, C. Orsi and L. Guidobono Cavalchini. 2008. Effects of Lactobacillus acidophilus D2/ CSL on laying hen performance. Italian Journal of Animal Science 7: 27-37.

14.Goodling, A. C. 1987. Production performance of white leghorn layers fed Lactobacillus fermentation products. Poultry science 66: 480-486.

15.Haddadin, M. S. Y., S. M. Abdulrahim, E. A. R. Hashlamoun  and R. K Robinson. 1996. The effects of Lactobacillus acidophilus on the production and chemical composition of hens eggs. Poultry science 75: 491-494.

16.Hajjaj, H., P. Duboc, L. B. Fay, I. Zbinden, K. Mace and P. Niederberger. 2005. Aspergillus oryzae products components inhibiting cholesterol biosynthesis downstream of dihydrolanostrol. FEMs Microbiol. Letters 242: 155-159.

17.Isshiki, Y. 1979. Effect of Lactobacilli in the diet on the concentration of nitrogenous compounds and minerals in blood of chickens. Japanese Poultry Science 16: 254-258.

18.Jensen, L. S., and D. V. Maurice. 1978. Effect of chromium and corn ferementation solubles on interior egg quality. Poultry Science 57: 1147-1151.

19.Jensen, L. S., C. H. Chang and S. P. Wilson. 1978. Interior egg quality: Improvement by distillers feeds and trace elements. Poultry Science 57: 448-454.

20.Jin, L. Z., Y. W., N. Abdullah. and S. Jalaludin. 1998. Growth performance, intestinal microbial population, and serum cholesterol of broilers fed diets containing lactobacillus. Journal of poultry Science 7: 1259-1265.

21.Jin, L. Z., Y. W. Ho, N. Abdullah and S. Jalaludin. 1997. Probiotics in poultry: Modes of action. Worlds Poultry Science 53: 351-368.

22.Kabir, S., M. M. Rahman, M. B. Rahman and S. U. Ahmad. 2004. The dynamics of probiotics on growth performance and immune response in broiler. Journal of Poultry Science 3: 61-64.

23.Kim, S. H., S. Y. Park, D. J. Yu,  S. J. Lee, K. S. Ryu and D. G. Lee. 2003. Effects of feeding Aspergillus oryzae ferments on performance, intestinal microflora, blood serum components and environmental factors in broiler. Korean Journal Poultry Science 30: 151-159.

24.Kurtoglu, V., F. Kurtoglu, E. Seker, B. Coskun, T. Balevi and E. S. Polat. 2004. Effect of probiotic supplementation on laying hen diets on yield performance and serum and egg yolk cholesterol. Food Additive Contamination 21(9): 817-823.

25.Lesson, S. and J. D. Summers. 1997. Commercial poultry nutrition, 3nd ed. University Books, Guelph, Ontario, Canada.

26.Mahdavi, A. H., H. R. Rahmani and J. Pourreza. 2005. Effect of probiotic supplements on egg quality and laying hens performance. International Journal of Poultry Science 4(7): 488-492.

27.Marks, H. L. and H. W. Washburn. 1991. Plasma and yolk cholesterol levels in Japanese quail divergently selected for plasma cholesterol response to adrenocoticotropin. Poultry Science 70: 429-433.

28.Miles, R. D. 1982. The protein sparing ability of Virginiamycin. Proceeding of 41st Annual Florida Poultry, 14-16.

29.Mohan, B., R. Kadirvel, M. Bhaskaran and A. Notarajan. 1995. Effect of probiotic supplementation on serum/yolk cholesterol and on egg shell thickness in layers. British Poultry Science 36: 799-803.

30.Nahashon, S. N., H. S. Nakaue and I. W. Mirosh. 1992. Effect of direct-fed microbials on nutrient retention and production parameters of laying pullets. Poultry Science 71(1): 111(Abstract).

31.Nahashon, S. N., H. S. Nakaue and I. W. Mirosh. 1996. Performance of single comb white leghorn fed a diet supplemented with a live microbial during the growth and egg laying  phases. Animal Feed Science and Technology 57: 25-38.

32.National Research Council. 1994. Nutrient requirements of poultry. 9th (ed.), Washington DC, National Academy Press, 44-45.

33.Qin, Z. R., T. Fukata, E. Baba and A. Arakawa. 1995. Effect of lactose and Lactobacillus acidophilus on the colonization on Salmonella enteritidis concurrently infected with Eimeria tenella. Avian Disease 39: 548-553.

34.SAS Institute. 1998. SAS User’s Guide: Statistics. SAS institute Inc., Cary, NC.

35.Sutton, C. D., W. M. Nuir and G. E. J. Mitchell. 1984. Cholestrol metabolism in laying hen as influenced by dietary cholesterol, caloric intake and genotype. Poultry Science 63: 972-980.

36.Thayer, R. H., R. F. Burkitt, R. D. Morrison and E. E. Murray. 1978. Efficiency of utilization of dietary phosphorus by caged Turkey breeder hens when fed rations supplemented with live yeast culture. Oklahoma State University, Stillwater, Animal Science Research 3: 15-22.

37.Tortuero, F., and E. Fernandez. 1995. Effect of inclusion of microbial culture in barley-based diets fed to laying hens. Animal Feed Science and Technology 53:255-265.

38.Washburn, K. W., and D. F. Nix. 1974. A rapid technique for extraction of yolk cholesterol. Poultry Science 53: 1118-1122.

39.Watkins, B. A., B. F. Miller, D. H. Neil and M. T. Collins. 1979. In vivo inhibitory effects of L. acidophilus against pathogenic E. coli in gnotobiotic chicks. Poultry Science 58: 1121(Abstract).

40.Yoon, C., C. S. Nam, J. H. Park, S. K. Han, Y. M. Nam and J. T. Kwon. 2004. Effect of feeding multiple probiotics on performance and fecal noxious gas emission in broiler chicks. Korean Journal Poultry Science 3: 229-235.