ارزیابی تنوع ژنتیکی در لاین های پیشرفته کلزای زمستانه

نوع مقاله: پژوهشی

نویسندگان

1 فرهیخته کارشناسی ارشد اصلاح نباتات دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران

2 استادیار مؤسسه تحقیقات اصلاح و تهیه نهال بذر کرج

3 استاد اصلاح نباتات دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران

چکیده

به منظور بررسی تنوع ژنتیکی، شناسایی و نیز انتخاب لاین­های برتر کلزا، تعداد 20 لاین پیشرفته در آزمایشی به صورت طرح بلوک‎های کامل تصادفی در سه تکرار برای صفات عملکرد و اجزای عملکرد در مزرعه موسسه تحقیقات اصلاح و تهیه نهال و بذر کرج در سال زراعی 88-1387 مورد بررسی قرار گرفتند. نتایج تجزیه واریانس نشان داد که بین لاین­ها برای تعداد روز تا گل‎دهی، پایان گل‎دهی و رسیدگی، طول دوره گل‎دهی، طول ساقه اصلی، ارتفاع اولین شاخه فرعی، طول خورجین اصلی، وزن هزار دانه، درصد روغن، عملکرد دانه و روغن، ارتفاع بوته، تعداد شاخه فرعی، تعداد خورجین در شاخه‎های فرعی و بوته اختلاف معنی­داری وجود دارد. برآورد وراثت­پذیری عمومی نشان داد که تعداد روز تا آغاز گل‎دهی و رسیدگی و وزن هزار دانه به ترتیب با مقدار 27/98، 52/92 و 73/91  گرم دارای بیشترین  مقدار وراثت­پذیری عمومی بودند تعداد خورجین در شاخه­های فرعی و بوته به ترتیب با 13/23 و 72/16 بیشترین ضریب تغییرات ژنوتیپی را نشان دادند. مقایسه میانگین عملکرد دانه نشان داد که لاین­های شماره 1، 9 و رقم شاهد زرفام به ترتیب با 49/3، 34/3 و13/3 تن در هکتار بالاترین عملکرد را داشتند. عملکرد روغن در سطح احتمال 1% همبستگی مثبت و معنی­دار، درصد روغن همبستگی مثبت و معنی‎دار در سطح احتمال 1% و طول خورجین فرعی همبستگی منفی و معنی­دار در سطح احتمال10% با عملکرد دانه داشتند. تجزیه خوشه­ای ژنوتیپ­های مورد مقایسه را به سه گروه متمایز دسته‎بندی نمود. یکی از این گروه­ها در برگیرنده لاین­های پرمحصول بود که از آن می­توان برای انتخاب لاین­هایی با عملکرد بالا در برنامه­های به‎نژادی بهره­برداری نمود.

کلیدواژه‌ها


عنوان مقاله [English]

Assessment of genetic variability in advanced lines of winter rapeseed

نویسندگان [English]

  • Leili Alizadeh 1
  • bahram alizadeh 2
  • farzad javidfar 2
  • farookh darvish 3
1 M.Sc. in Plant Breeding, Science and Research Branch, Islamic Azad University,
2 Assistant Prof., Oilseeds Research Department, Seed and Plant Improvement Institute (SPII), Karaj-Iran
3 Professor in Plant Breeding, Science and Research Branch, Islamic Azad University,
چکیده [English]

   In order to study the genetic variation, identification and selection of superior rapeseed lines in a F4 generation, 20 advanced lines were examined using a randomized complete block design with three replications in the field of Seed and Plant Improvement Institute (SPII), Karaj in 2008-2009. Results of analysis of variance showed that there were significant differences between genotypes for the number of days to initiating and terminating of flowering, number of days to ripening, main stem length, height of first branch from ground surface, main pod length, 1000 seed weight, oil content, seed and oil yield, plant height, number of primary branches, number of pods per branch and per plant. Number of days to beginning of flowering and ripening, 1000 seed weight with 98.27, 92.52 and 91.73 grams had the highest values for broad sense heritability, respectively. The traits like number like of pods per branch and per plant with 23.13 and 16.72 had the highest values for genetic coefficient of variation (GCV). Comparison of mean seed yield showed that the genotypes L1, L9 and Zarfam with the yield values of 3.49, 3.34 and 3.13 t/ha had the highest performance among the examined genotypes, respectively. Correlation among the measured traits showed that oil yield had a positive and significant correlation with seed yield at 1% of probability level and the traits of oil percent and the secondary pod length showed negative and significant correlation with seed yield at the %10 of probability level. Based on cluster analysis, genotypes were assigned into three distinct groups. One of the resulting clusters consisted of high yielding lines that would be suitable for selection of superior and high yielding rapeseed varieties.

کلیدواژه‌ها [English]

  • Broad sense heritability
  • yield
  • Components of yield
  • Genetic variance
  • Rapesees
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