نوع مقاله : پژوهشی
نویسندگان
1 دانشجوی سابق کارشناسی ارشد گروه اصلاح نباتات دانشکده کشاورزی دانشگاه تبریز تبریز، ایران
2 استادان گروه زراعت و اصلاح نباتات دانشکده کشاورزی دانشگاه تبریز تبریز، ایران
3 استادیار موسسه اصلاح و تهیه نهال و بذر کرج، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
In present research, genetic diversity of gliadins among 30 wheat landraces belong to region of Zanjan-Iran, was studied by Acid-PAGE technique. Totally 54 polymorphic gliadin bands were identified. The highest frequency was related to gliadin band 46 witch observed in 90% of wheats. Also gliadins 12.5 and 15.5 were observed in 86.7% of the wheats. Genetic diversity of the wheats based on total gliadin found to be high (H=0.967). Highest and Lowest genetic variation related to omega-gliadins (H=0.917) and gamma-gliadins (H=0.781) respectively. Wheats of Zanjan showed diversity in terms of gliadin bands number (11-25 bands). The entire wheats, showed specific gliadin pattern. Totally, 29 omega-gliadin patterns, 15 gamma-gliadin patterns, 22 beta-gliadin patterns and 23 alpha-gliadin patterns were observed. Cluster analysis could separate three tetraploid genotypes TN-11372, TN-11734, TN-11743 from hexaploids. These results indicate that gliadin proteins analysis is useful for evaluating of genetic diversity, characterizing of wheat genotypes and separating of different ploidy levels.
کلیدواژهها [English]
In present research, genetic diversity of gliadins among 30 wheat landraces belong to region of Zanjan-Iran, was studied by Acid-PAGE technique. Totally 54 polymorphic gliadin bands were identified. The highest frequency was related to gliadin band 46 witch observed in 90% of wheats. Also gliadins 12.5 and 15.5 were observed in 86.7% of the wheats. Genetic diversity of the wheats based on total gliadin found to be high (H=0.967). Highest and Lowest genetic variation related to omega-gliadins (H=0.917) and gamma-gliadins (H=0.781) respectively. Wheats of Zanjan showed diversity in terms of gliadin bands number (11-25 bands). The entire wheats, showed specific gliadin pattern. Totally, 29 omega-gliadin patterns, 15 gamma-gliadin patterns, 22 beta-gliadin patterns and 23 alpha-gliadin patterns were observed. Cluster analysis could separate three tetraploid genotypes TN-11372, TN-11734, TN-11743 from hexaploids. These results indicate that gliadin proteins analysis is useful for evaluating of genetic diversity, characterizing of wheat genotypes and separating of different ploidy levels.