القای مقاومت گندم به بیماری پاخوره با کاربرد متیل جاسمونات و چند گونه قارچ میکوریز

نوع مقاله : پژوهشی

نویسندگان

گروه بیماری شناسی گیاهی واحد شیراز دانشگاه آزاد اسلامی شیراز، ایران

10.22034/aej.2017.528713

چکیده

بیماری پاخوره یکی از مهم­ترین بیماری­های گندم می ­باشد که توسط قارچ خاکزی Gaeumannomyces graminis ایجاد می ­شود. این پژوهش با هدف تعیین تأثیر گونه­ های قارچ میکوریز و متیل جاسمونات در القای مقاومت گندم آلوده به قارچ مذکور انجام شد. آزمایش در قالب طرح کاملاً تصادفی با آزمایش فاکتوریل با 32 تیمار در سه تکرار در شرایط گلخانه انجام گرفت. فاکتور اول وجود و عدم وجود بیمارگر، فاکتور دوم گونه ­های قارچ میکوریز شامل Glomus hoi، G. mosseae و G. intraradices و فاکتور سوم غلظت­ های متیل جاسمونات در مقادیر 0، 1/0، 5/0 و 5/1 میلی ­مولار بود. عوامل القا کننده مقاومت فوق­ الذکر در مرحله گیاهچه به خاک اطراف ریشه اضافه و در بازه­ های زمانی 24 و 120 ساعت میزان فنل اندازه­ گیری و روند تغییرات آن مورد بررسی قرار گرفت. میزان فعالیت فنل کل در اکثر تیمارهای سالم نسبت به تیمارهای بیمار القا شده بیشتر بود. میزان فنل کل در گیاهان بیمار با هر عامل القای مقاومت در بازه زمانی 120 ساعت بیشتر از میزان آن در بازه زمانی 24 ساعت بود ولی به طور کلی گیاهان سالم بیشترین میزان فنل کل در دو بازه زمانی 24 و 120 ساعت را داشتند. کاربرد G. intraradices همراه با غلظت 5/1 میلی­ مولار متیل جاسمونات بیشترین تأثیر را در القای مقاومت داشت و پیشنهاد می­ شود از این ترکیب در مرحله گیاهچه به عنوان کود بیولوژیک جهت القای مقاومت برای مقابله با بیماری پاخوره گندم استفاده شود.

کلیدواژه‌ها

عنوان مقاله [English]

Induced resistance in take-all infected wheat using methyl jasmonate and Glomus spp.

نویسندگان [English]

  • Ahmad Baneshi
  • Seddiqe Mohammadi
Department of plant pathology Shiraz Branch Islamic Azad University Shiraz, Iran
چکیده [English]

Take-all is one of the most important wheat soil-borne diseases caused by Gaeumannomyces graminis. The research objective was determination of Glomus spp. and methyl jasmonate effect on resistance induction of wheat take-all infected seedlings.The experiment was carried out based on completely randomized design in factorial with 32 treatments and three replications in greenhouse condition.The factors were presence or absence of pathogen, mycorrhizal fungi species including Glomus intraradices, G. mosseaeand G. hoi inoculation and methyl-jasmonate added in four concentrationsof 0, 0.1, 0.5 and 1.5mM. Induced resistance factors were added to surrounding soil in seedling stage and total phenol were measured after 24 and 120 hours inoculation.Total phenolactivity in healthy plants was more than infected and induced plant in most of the treatments. Total phenol in infected seedling after 120 hours was more than in 24 hours with all induced resistance factor. However, healthy seedlings had more total phenol after 24 and 120 hours. Application of G. intraradicesalong with 1.5mM of methyl jasmonate had the greatest impact on induced resistance. Therefore, it is recommended to be used as fertilizer for resistance inducing to take all disease in wheat.

کلیدواژه‌ها [English]

  • o biological control
  • o induced resistance
  • o mycorrhizal fungi

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