شناسایی گونه های فوزاریوم مرتبط با گندم، جو و ذرت در استان آذربایجان غربی

نوع مقاله: پژوهشی

نویسندگان

1 عضو هیات علمی دانشگاه ازاد اسلامی واحد مهاباد, گروه گیاهپزشکی

2 گروه گیاهپزشکی دانشگاه ارومیه ارومیه، ایران

10.22034/aej.2017.528886

چکیده

جنس فوزاریوم از قارچ‌های خاکزی است که اهمیت اقتصادی زیادی داشته و شامل گونه‌های بیماری‌زای گیاهی است که دامنه وسیعی از گیاهان از جمله گندمیان را آلوده می‌کنند. در این پژوهش، تنوع فوزاریوم‌های مرتبط با گندم، جو و ذرت، در استان آذربایجان غربی بطور مرفولوژیکی مورد بررسی قرار گرفت.نمونه‌های آلوده و مشکوک ریشه، طوقه و ساقه از مزارع محصولات مذکور جمع­آوری گردید. برای جداسازی از محیط‌های کشت سیب زمینی دکستروز آگار، محیط کشت نش و اسنایدر و محیط دیکلران کلرامفنیکل پپتون آگار استفاده شد. پس از خالص­سازی برای وادارسازی برخی جدایه‌ها به اسپورزایی و تولید اسپور‌های تیپیک، زنجیر کنیدیوم و کلامیدوسپور از محیط‌های کشت­های متنوعی استفاده شد. شناسایی جدایه‌ها بر اساس ویژگی‌های ریخت­شناسی و ریخت­سنجی آنها در محیط­های کشت و شرایط خاص و با استفاده از کلید‌های شناسایی معتبر صورت گرفت. در این بررسی 11 گونه از جنس فوزاریوم شامل Fusarium acuminatum، F. bactridioides،F. chlamydosporum، F. compactum ، F. coeruleum، F. culmorum، F.flocciferum، F. nygamai ، F. oxysporum، F. polyphialidicum  و F. solani  شناسایی گردید. بیشترین تنوع گونه­های شناسایی شده مربوط به جو و سپس به ترتیب گندم و ذرت بود.  گونه­های F. polyphialidicum، F. bactridioidesو F. coeruleum برای اولین بار از ایران گزارش می­شوند.

کلیدواژه‌ها


عنوان مقاله [English]

Identification of Fusarium species associated with wheat, barley and corn in West Azarbaijan Province, Iran

نویسندگان [English]

  • Nabi Khezrinejad 1
  • Younes Rezae Danesh 2
1 Academic staff, member of plant protection department, IAU-Mahabad
2 Department of Plant Protection Mahabad Branch Islamic Azad University Mahabad, Iran
چکیده [English]

Fusarium is the soil born fungus which is very economically important and includes several plant pathogenic species infecting a wide range of different plants including geramineous ones. In this research, diversity of Fusarium species associated with wheat, barley and corn was studied morphologically. The infected or suspected crown, root and stem of plants were collected from different area of West Azarbaijan Province, Iran. For isolation, the growth media of PDA, NS and DCPA were used. After purification, to make sporulation, microconodium chain and chlamydospore production, different media cultures were used. Identification was conducted using morphological and morphometrical characteristics on especial media cultures and conditionsusing diagnostic keys. Totally, 11species including Fusariumacuminatum, F. bactridiodes, F. chlamydosporum, F. compactum, F. culmorom, F. coeruleum, F. flocciferum,F. nygamai, F. oxyporum, F. polyphialidicum, and F. solaniwere identified. The most species diversity belonged to barley, wheat and corn, respectively. F. polyphialidicum, F. bactridiodesand F. coeruleumare new reports for mycoflora of Iran.

کلیدواژه‌ها [English]

  • o biodiversity
  • o fungal flora
  • o mycoflora
  • o taxonomy
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