پایداری فیتوشیمیایی مرزه در شرایط نگهداری فراسرد

نوع مقاله: پژوهشی

نویسندگان

1 گروه علوم باغبانی، دانشکده کشاورزی، واحد رودهن، دانشگاه آزاد اسلامی، رودهن، ایران

2 مؤسسه تحقیقات جنگل‌ها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

10.22034/aej.2017.537586

چکیده

به منظور مقایسه نوع و میزان ترکیبات فیتوشیمیایی مرزه به عنوان یک گونه‌ ارزشمند، بومی و در خطر انقراض ایران، در شرایط فراسرد، بذور مرزه به مدت 1 هفته در نیتروژن ‌مایع با دمای 196- درجه سلسیوس نگهداری شدند. اسانس‌گیری از سرشاخه‌های گلدار حاصل از این بذور به روش تقطیر با آب انجام گرفت و ترکیبات تشکیل‌دهنده اسانس‌ با روش کروماتوگرافی ‌گازی و کروماتوگرافی ‌گازی ‌متصل به طیف‌سنج‌ جرمی شناسایی شد. تعداد 15 و 16 ترکیب به ترتیب از اسانس سرشاخه‌های مرزه حاصل از بذور نگهداری شده در شرایط فراسرد و معمولی شناسایی شد و ترکیبات عمده شامل کارواکرول، پاراسیمن و تیمول بودند. از لحاظ نوع و درصد ترکیبات موجود در اسانس تفاوت قابل توجهی بین گیاهان شاهد و فراسرد مشاهده نشد. بنابراین، با استفاده از فناوری فراسرد می ­توان بذر این گونه ارزشمند را برای مدت زمان بسیار طولانی حفظ و از خطر انقراض این گونه منحصر ‌به ‌فرد جلوگیری نمود.

کلیدواژه‌ها


عنوان مقاله [English]

Phytochemical persistence of Satureja rechingeri under cryopreservation conditions

نویسندگان [English]

  • Shabnam Shahbazi 1
  • Fatemeh Sefidkon 2
  • Abbas Ghamari Zare 2
1 Department of Horticulture, Roudehen Branch, Islamic Azad University, Roudehen , Iran
2 Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension organization, Tehran, Iran
چکیده [English]

This research was aimed to compare the type and content of phytochemical compounds of Satureja rechingeri as a valuable, indigenous and endangered species of Iran under cryopreservation. The seeds were kept in liquid nitrogen at -196 °C for 1 week. The flowering shoots resulted from the treated seeds were collected and dried in the laboratory condition and their essential oils were obtained by hydro-distillation method. The essential oil compositions were determined using analytical gas chromatography and gas chromatography coupled with mass spectrometer (GC/MS). Finally, 15 and 16 componentswere identified in the essential oils in the cryopreservation and control groups, respectively. The main components in the essential oil were carvacrol, p-cymeneand and thymol. Regarding the type and percentage of compounds existing in the essential oil, there was no significant difference among the control and cryopreservation treatments. Therefore by cryopreservation technique, the seeds of this valuable endangered species could be preserved for a long period and its extinction may be avoided.

کلیدواژه‌ها [English]

  •  essential oils
  •  long term storage
  •  plant germplasm
  •  ultra cold
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