اثر حشره‌کش‌های میکروبی و شیمیایی بر سنین مختلف لاروی سوسک کلرادوی سیب‌زمینی در شرایط آزمایشگاهی

نوع مقاله: پژوهشی

نویسندگان

1 گروه آگرواکولوژی، واحد مهاباد، دانشگاه آزاد اسلامی، مهاباد، ایران

2 گروه گیاهپزشکی، دانشکده کشاورزی و منابع طبیعی، واحد مهاباد، دانشگاه آزاد اسلامی، مهاباد، ایران

10.22034/aej.2018.539455

چکیده

در این پژوهش، حساسیت سنین مختلف لاروی سوسک کلرادوی سیب زمینی به فرآورده تجارتی بایوبیت محتوی باکتری Bacillus thuringiensis var. tenebrionis به عنوان یک آفت­کش میکروبی و حشره‌کش­های شیمیایی ایمیداکلوپراید و فوزالون به ترتیب به عنوان حشره‌کش رایج و جدید برای مهار این آفت، در شرایط آزمایشگاهی مورد بررسی قرار گرفت. بدین منظور سوسک کلرادوی روی برگ‌های سیب‌زمینی در شرایط گلدانی پرورش داده شده و حساسیت سنین لاروی اول، دوم، سوم و چهارم در آزمایش­­های زیست‌سنجی مشخص گردید. مقادیر غلظت­های 50% کشندگی بایوبیت بعد از 144 ساعت برای سنین مختلف لاروی مذکور به ‌ترتیب 252، 613، 1731 و 3006 و برای ایمیداکلوپراید بعد از 72 ساعت به‌ترتیب 58/17، 35/41، 76/56 و 27/81 و برای فوزالون در همین زمان به ترتیب 447، 698، 1020 و 1297 قسمت در میلیون برآورد شد. با افزایش سنین لاروی مقاومت لاروها نسبت به تیمار به کار برده شده افزایش یافت. همچنین، سوسک کلرادوی سیب زمینی نسبت به ایمیداکلوپراید حساس‌تر از فوزالون و بایوبیت و نسبت به فوزالون حساس‌تر از بایوبیت بود که می­تواند در مهار شیمیایی این آفت مورد توجه قرار گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of microbial and chemical pesticides on different instars larvae of Colorado potato beetle in laboratory conditions

نویسندگان [English]

  • Shirin Mohammadzadeh 1
  • Akbar Ghassemi-Kahrizeh 2
1 Department of Agroecology, Mahabad Branch, Islamic Azad University, Mahabad, Iran
2 Department of Plant Protection, Faculty of Agriculture, Mahabad Branch, Islamic Azad University, Mahabad, Iran
چکیده [English]

In this study, the susceptibility of different larval instars of Colorado potato beetle, Leptinotarsa decemlineata to Biobit containing Bacillus thuringiensis var. tenebrionis Ber., as microbial and imidacloprid  as novel chemicals and phosalone as conventional insecticides was investigated in laboratory conditions. Colorado potato beetles were propagated on potato leaves planted in pots and their 1st, 2nd, 3rd, and 4th larval instars’ susceptibility was determined by bioassay test. LC50 values of Biobit for L1, L2, L3 and L4 were defined as 252, 613, 1731 and 3006 ppm after 144 hours, respectively. Also, LC50 values for imidacloprid and phosalone after 72 hours were 17.58, 41.35, 56.76 and 81.27 ppm and 447, 698, 1020 and 1297 ppm, respectively. Older insect larvae were more resistant to microbial and chemical treatments.  Also, Colorado potato beetle was more susceptible to imidacloprid then phosalone and Biobit.

کلیدواژه‌ها [English]

  •  biopesticide
  •  chemical control
  •  imidacloprid
  •  microbial control
  •  phosalone
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