‌اثرات ضدقارچی عصاره متانولی بومادران، آویشن، چای‌کوهی و پیاز کوهی بر کپک پنی‌سیلیومی مرکبات در شرایط آزمایشگاهی

نوع مقاله: پژوهشی

نویسندگان

1 گروه بیماری‌شناسی گیاهی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

2 گروه بیماری‌شناسی گیاهی، واحد مرند، دانشگاه آزاد اسلامی، مرند، ایران

10.22034/aej.2018.543119

چکیده

وقفه طولانی بین زمان برداشت و مصرف مرکبات باعث ایجاد ضایعات زیادی از طریق ابتلا به بیماری­ های قارچی از جمله کپک سبز مرکبات ناشی از Penicillium digitatum  می­ شود. یکی از روش­ های نوین مهار بیماری های پس از برداشت استفاده از عصاره های گیاهی است. این پژوهش برای ارزیابی فعالیت ضد قارچی عصاره چهار گیاه دارویی بومادران، آویشن، چای کوهی و پیاز کوهی در سطح آزمایشگاهی بر کپک سبز مرکبات انجام شد. عصاره گیری با استفاده از متانول با دستگاه دکانتور انجام شد. بررسی اثر بازدارندگی عصاره ­ها در رقت­ های 25/0، 5/0، 75/0 و 1 در هزار روی رشد میسیلیومی و اسپورزایی قارچ در محیط کشت سیب زمینی دکستروز آگار در قالب طرح فاکتوریل بر پایه کاملاً تصادفی انجام شد. عصاره آویشن و پیاز کوهی در غلظت­ های 1 در هزار، به ترتیب 100 و 28% بر رشد میسیلیومی بازدارنده بودند ولی عصاره بومادران و چای کوهی در این غلظت فاقد اثر بازدارندگی بودند. همچنین عصاره  پیاز کوهی و آویشن در غلظت 1 در هزار به طور کامل بر اسپورزایی قارچ بازدارنده بودند. عصاره آویشن و پیاز کوهی در غلظت 1 در هزار گزینه ­های مناسبی می ­توانند برای مهار کپک پنیسیلیومی مرکبات در شرایط انباری باشند.

کلیدواژه‌ها


عنوان مقاله [English]

Antifungal effects of some herbal extracts on Penicillium mould of citrus in laboratory conditions

نویسندگان [English]

  • Somayeh Gholchinno 1
  • Behnam Pouzeshimiyab 2
  • Sevil Nematollahi 1
1 Department of Plant Pathology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 Department of Plant Pathology, Marand Branch, Islamic Azad University, Marand, Iran
چکیده [English]

Long time interval between harvesting and the consumption of citrus causes a lot of waste through fungal diseases, including citrus green mould, by Penicillium digitatum. One of the most recent methods to control post-harvest diseases is the use of plant extracts. This research was conducted on the antifungal activity of four herbs of Achillea millefolium, Allium schoenoprasum, Stachys lavandulifolia and Thymus vulgaris on citrus green mould in laboratory level. Plant extract was done using methanolic extracts. The inhibitory effect of extracts at concentration 250, 500, 750 and 1000 ppm on the mycelium growth and conidia germination of fungi was carried out in potato- dextrose- Agar culture medium. This experiment was studied in a completely randomized factorial model. The results showed that the effect of some herbal extracts on the percentage control of citrus greenmould was significantly different at .01 probability level, so that the extracts of Thyme and chive in concentrations of 1000 ppm had inhibitory growth of 100 and 28.06 percent respectively. Also, it was determined that, methanolic extracts of chive and thyme completely inhibited germination of P. digitatum conidia in concentration of 1000 ppm. The results of the study showed that the extracts of thyme and chive in respective concentration of 1000 ppm are good choices for studying the control of Penicillium mould in storage conditions.

کلیدواژه‌ها [English]

  • mycelium inhibition
  • natural control
  • Penicillium digitatum
  • plant extract
  • spore inhibitor
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