پاسخ‌های فیزیولوژیک و تغییرات پروتئین حرارتی در سرخارگل تحت تنش خشکی

نوع مقاله : پژوهشی

نویسندگان

گروه باغبانی، دانشکده کشاورزی، واحد خوراسگان، دانشگاه آزاد اسلامی، اصفهان، ایران

10.22034/aej.2018.545685

چکیده

سلول‌‌های گیاهی جهت درک سیگنال‌‌های مختلف پیرامون­ شان نمو یافته‌‌اند و از طریق تعدیل بیان ژن‌‌ها به آن‌‌ها پاسخ می‌‌دهند. خشکی یک ویژگی آب و هوایی طبیعی و محدود کننده‌ عملکرد گیاهان زراعی است. در این پژوهش به ‌منظور ارزیابی واکنش گیاه سرخارگل به تنش خشکی، در یک آزمایش گلدانی، پس از اعمال سطح تنش خشکی شامل آبیاری به میزان 25، 50، 75 و 85% ظرفیت زراعی همراه با آبیاری معمول، میزان پرولین، پتاسیم، فسفر و نیتروژن برگ اندازه­ گیری و نیز بیان پروتئین­ های شوک حرارتی با استفاده از روش Real- Time PCR در بافت‌ برگ مورد ارزیابی قرار گرفت. افزایش معنی‌دار میزان پرولین، کاهش پتاسیم، فسفر و  نیتروژن در بافت‌ برگ در اثر اعمال تنش خشکی مشاهده شد. همچنین اعمال تنش خشکی موجب افزایش بیان پروتئین شوک حرارتی در تمامی سطوح تنش مورد مطالعه شد. به طور کلی، گیاه سرخارگل با به کارگیری برخی سازوکارهای دفاعی از قبیل افزایش محتوی پرولین و پروتئین شوک حرارتی در مقابل تنش خشکی مقاومت می ­کند.

کلیدواژه‌ها

عنوان مقاله [English]

Physiological reactions and thermal protein dynamics in coneflower under drought stress

نویسندگان [English]

  • Kosar Moradi
  • Fariba khalili
Department of Horticulture, Faculty of Agriculture, Khorasgan Branch, Islamic Azad University, Isfahan, Iran
چکیده [English]

Plant cells have evolved to understand the various signals in their surroundings and respond to them by modulating the expression of genes. Drought is a natural and recurrent climatic feature in most parts of the world and plays an important and limiting role in crop yields. In this study, to ensure the stress on the medicinal herb of coneflower (Echinacea purpurea), the proline, potassium, phosphorus and nitrogen content of the leaves were evaluated in a completely randomized design with three replications, each of which was repeated in three pots. Also, expression of heat shock proteins in leaf tissue under four levels of drought stress irrigation at 25%, 50%, 75% and 85% of crop capacity was evaluated. The results showed a significant increase in the amount of proline, potassium, phosphorus and nitrogen in leaf tissue. Also, examination of thermal shock protein expression using Real-Time PCR indicated that drought stress significantly increased expression of heat shock protein in all studied treatments, which also proved the changes caused by stress.  In general, the coneflower plant resists some degree of resistance using of some protective mechanisms, such as increasing proline and heat shock proteins content.

کلیدواژه‌ها [English]

  •  Echinace purpurea
  •  heat shock protein
  •  Abiotic stress
  •  Prolin

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