کارآیی روش‌های استخراج RNA از برگ گیاه دارویی سرخارگل

نوع مقاله : پژوهشی

نویسندگان

1 بخش تحقیقات سلولی مولکولی، مدیریت بیوتکنولوژی کشاورزی منطقه مرکزی کشور- اصفهان، پژوهشکده بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات،

2 بخش تحقیقات ژنومیکس، مدیریت بیوتکنولوژی کشاورزی منطقه مرکزی کشور- اصفهان، پژوهشکده بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران

10.22034/aej.2019.665295

چکیده

سرخارگل گیاه دارویی ارزشمند حاوی سطوح بالایی از متابولیت­های ثانویه می­باشد که تا کنون روش مناسبی جهت استخراج RNA کل از آن معرفی نشده است. در پژوهش حاضر چهار روش استخراج RNA شامل روش لیتیم کلراید، کیت RNAX-Plus، فنل/SDS و لیتیم کلراید/فنل مورد بررسی قرار گرفت. به منظور تأیید کیفیت و خلوص RNA از روش اسپکتروفتومتری و الکتروفورز روی ژل آگارز 5/1% استفاده شد. نسبت بالای جذب محلول RNA در طول‌موج 260 نانومتر به طول موج 280 و 230 نانومتر، مشاهده RNA های ریبوزمی و عدم مشاهده اسمیر روی ژل به عنوان معیاری برای تعیین کیفیت نمونه‌ها در نظر گرفته شد.  بالاترین کیفیت RNA حاوی 9/2 میکروگرم بر میکرولیتر در 100 میلی‌گرم برگ تازه در روش فنل/SDS و کمترین کیفیت RNA حاوی 2/1 میکروگرم در 100 میلی‌گرم برگ تازه در روش لیتیم کلراید/فنل مشاهده شد. بنابراین، روش فنل/SDS به عنوان مناسب­ترین روش برای استخراج RNA برگ گیاه سرخارگل معرفی می­گردد

کلیدواژه‌ها

عنوان مقاله [English]

Efficiency of RNA extraction methods from coneflower medicinal plant

نویسندگان [English]

  • Kosar Moradi 1
  • Rasol Amirian 2
1 Department of Molecular cell, Agricultural Biotechnology Research Institute of Iran-Central Branch- Isfahan, Agricultural Research,Education and Extension Organization (AREEO), Tehran, Iran
2 Department of Genomics, Agricultural Biotechnology Research Institute of Iran-Central Branch- Isfahan, Agricultural Research, Education and Extension Organization (AREEO), Tehran
چکیده [English]

Coneflower is a valuable medicinal plant containing high levels of secondary metabolites, but no suitable method for extraction of the entire RNA has been introduced yet. In present study, four methods of RNA extraction including lithium chloride, RNAX-Plus kit, phenol/SDS and lithium chloride/phenol were investigated. For confirming the quality and purity of RNA, spectrophotometer and agarose gel electrophoresis of 1.5% were used. The high absorption ratio of RNA solution at a wavelength of 260 nM to 280 and 230 nM, observation of ribosomal RNAs and the lack of smear on the gel were considered as criteria for determining samples quality. The highest and the lowest qualities of RNA contained 2.9 μg/100 mg of fresh leaves by phenol/SDS method and 1/2 μg/100 mg of fresh leaves by lithium chloride/phenol method, respectively. Therefore, phenol/SDS method is introducing as the most suitable method for RNA extraction from coneflower.

کلیدواژه‌ها [English]

  • Echinacea purpurea
  • lithium chloride/phenol
  • litium chloride
  • phenol/SDS
  • RNAX-Plus kit

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