اثر پیری کنترل شده بر جوانه‌زنی، فعالیت بیوشیمیایی و آنزیمی گندم سیاه

نوع مقاله: پژوهشی

نویسندگان

1 دانشکده کشاورزی دانشگاه شاهد

2 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه شاهد، تهران، ایران

10.22034/aej.2019.1872308.1101

چکیده

به منظور بررسی اثر پیری کنترل شده بر جوانه­زنی، فعالیت بیوشیمیایی و آنزیمی گندم سیاه یک آزمایش به صورت فاکتوریل در قالب طرح کاملاً تصادفی در آزمایشگاه علوم و تکنولوژی بذر دانشگاه شاهد اجرا شد. تیمارهای آزمایش شامل مدت زمان پیری 2، 4 و 7 روزه و محتوای رطوبت بذر 10، 20 و 30% بود. صفاتی از قبیل درصد جوانه­زنی، مدت زمان جوانه­زنی، هدایت الکتریکی، پراکسیداسیون لیپید و فعالیت آنزیم کاتالاز اندازه­گیری شد. اثر مدت زمان پیری، محتوای رطوبتی بذر و برهمکنش آنها بر صفات فوق آزمایش معنی­دار بود. بالاترین میزان جوانه­زنی و فعالیت آنزیم کاتالاز در بذرهای پیر شده به مدت 2 روز با محتوای رطوبتی 20% مشاهده شد. مدت زمان جوانه­زنی، هدایت الکتریکی و پراکسیداسیون لیپید در بذرهای پیر شده به مدت 7 روز با محتوای رطوبتی 30% به بیشترین مقدار خود رسید. همچنین، با افزایش محتوای رطوبتی و مدت زمان پیری بذر، سازوکارهای دخیل در پیری بذر افزایش یافته و با تأثیر بر فسفولیپیدهای غشای سلولی افزایش هدایت الکتریکی و پراکسیداسیون لیپید و کاهش جوانه­زنی و فعالیت آنزیم کاتالاز مشاهده شد. بنابراین، توصیه می­شود برای جلوگیری از زوال بذرهای گندم سیاه در ذخیره­سازی طولانی مدت و کوتاه مدت میزان محتوای رطوبتی %10 باشد. 

کلیدواژه‌ها


عنوان مقاله [English]

Effect of seed deterioration on germination, biochemical and enzymatic activity of buckwheat

نویسندگان [English]

  • Saeideh Maleki Farahani 2
  • Shokofeh Gholami 2
1 College of Agriculture, Shahed University
2 Department of Crop Production and Plant Breeding, Shahed University, Tehran,
چکیده [English]

 To determine the effect of seed deterioration on germination, biochemical and enzymatic activity of buckwheat, an experiment was conducted as factorial based on completely randomized design in Seed Science and Technology Laboratory of Shahed University, Iran. The treatments consisted of ageing duration (non-aging, 2, 4 and 7 days) and seed moisture content (10, 20 and 30%). Parameters such as germination percentage, mean germination time, electrical conductivity, lipid peroxidation and catalase activity were measured. The effect of aging duration, seed moisture content and their interaction on traits were significant. Germination percentage, mean germination time, electrical conductivity, lipid peroxidation, and catalase enzyme activity were significantly affected by aging duration and seed moisture content. The highest germination percentage and catalase enzyme activity in aged seeds was observed during two days with 20% moisture content. Mean germination time, electrical conductivity and lipid peroxidation in aged seeds for seven days was in the highest level in seeds with 30% moisture content. Seed moisture content and aging duration increased the mechanisms involved in seed aging and cell membrane phospholipids affected by deterioration, which increased the electrical conductivity and lipid peroxidation and reduced germination and catalase enzyme activity. To prevent buckwheat seed deterioration in long and short term storage, it is recommended that seed moisture content is kept about 10%.

کلیدواژه‌ها [English]

  •  catalase enzyme activity
  •  electrical conductivity
  •  Fagopyrum esculentum
  •  lipid peroxidation
  •  seed deterioration
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